Objective: We investigated whether autogenic training (AT) altered resting state functional connectivity. Methods: 7 volunteers familiar with AT participated in this study. Rs-fMRI was performed pre and post AT, and 3D-T1WI was acquired. Group analysis was performed to explore the alteration of resting functional connectivity after autogenic training by using CONN functional connectivity toolbox. Results: Greater connectivity between 1. right amygdala and right caudate, 2. precuneus and left pallidum, and 3. right supratemporal gyrus and left pallidum, were demonstrated. Conclusion: AT could have positive effects not only to restore the automatic nervous system but also to alter RS functional connectivity.