We aimed to develop a non-invasive quantitative MRI methodology to assess positional and rotational impact on the airway opening in subjects with history of sleep apnea for a proposed clinical trial. An equipment and software analysis approach was implemented to perform a feasibility demonstration using 9 volunteers. All data analysis was performed on IntelliSpace Portal (ISP) (Philips Healthcare) to validate the angles and perform the assessment of the airway opening in the RP and RG regions. We have established a prototype table setup that enables us to image 7 positions of various head and chest support angles to investigate the best positional changes to improve the airway opening of sleep apnea subjects. The results showed that the actual angles were within 0.10-0.30 from the targeted angles. In conclusion, the developed 3T MRI methodology appears robust to assess the impact of head and chest positional changes on the airway in sleep apnea patients.