Magnetization transfer (MT) imaging and amide proton transfer (APT) imaging have reported many promising results. However, little is known about their usefulness for prostate cancer (PCa). In this study, MT-APT imaging were performed for 39 patients with pathological proven PCa. The feasibility of MT imaging and APT imaging for PCa detection was assessed, and their differential diagnostic values for PCa were compared. The results revealed that MTR(16.5 ppm) increased in cancerous tissues compared with normal PZs, and MT imaging outperformed APT imaging for PCa diagnosis. MT imaging showed promising role in the diagnosis of PCa.