Whether there exists a lag in the iron loading between liver and heart in transfusion dependent patients has clinical significance in prevention and treatment of heart iron overload. We performed this study to verify this time lag on a rabbit model. The result shows that the iron loading, measured with pathology, MRI and atomic absorption spectrophotometer, was much faster and heavier in the liver than that in the heart. Therefore, there existed a time lag between heart and liver iron overload on the rabbit model. This may lead to improved clinical guidelines for cardioprotection.