We performed a regional analysis of hyperpolarized 129Xe gas-uptake parameters in the lung using localized chemical shift saturation recovery spectroscopy and dissolved-phase imaging with a three-point Dixon method for comparison. Localization of spectroscopic data acquired without spatial encoding was achieved using a 16-channel receive coil and Spectral Localization Achieved by Sensitivity Heterogeneity (SPLASH). The reproducibility of all parameters was studied and coefficients of variation are reported. Localized CSSR data exhibit evidence of gravitational effects in consistency with dissolved-phase imaging. The septal wall thickness derived from evaluation of CSSR data using the Patz model can be determined regionally with high reproducibility.