Imbalance in dietary intake and energy expenditure are associated with obesity, diabetes and metabolic disorders. Adipocyte size and expansion of adipose tissue plays a critical role towards the progression of diet induced obesity. Brown adipose tissue (BAT) plays a critical role in modulating different fat depots in the body. BAT can be functionally activated by administering the β3-adrenergic agonist. Understanding the mechanisms associated with BAT activation and the possibility of reversing insulin resistance and its impact on whole body metabolism is of current clinical interest for combating diabetes. In the current study, we investigated the fat partitioning in high fat diet induced obese rodent model by β-adrenergic-mediated BAT activation.