The relative contributions of the four compartments of the quadriceps to force production are clinically very important information. The spatial and temporal heterogeneity of velocity and strain, (surrogate biomarkers of neural activation) was determined, within and between different compartments of normal quadriceps and along the proximo-distal (Z) axis, during isometric contraction using gated VE-PC imaging. Statistically significant differences were determined, within the same muscle compartment, across compartments and between different Z axis positions. Determining how these change in the diseased state e.g. post-ACL tear will be important in tailoring rehabilitative strategies, with particular relevance to preventing early onset of osteoarthritis.