In a test-retest fMRI experiment we examined the influence of temporal filtering and global signal regression (GSR) on resting state networks in rats. Connectivity and topological properties as well as their test-retest reliability were assessed for eight filtering variants (with and without GSR, four frequency bands). We found GSR to strongly impair the expected structure of networks. The choice of temporal filtering frequencies whereas did not have a significant effect. Test-retest-reliability was low for all filtering variants. Based on our results we recommend to use less restrictive bandpass filters but no GSR.