The challenges in T2 measurement of lactate (Lac) in healthy brain tissue include J-modulation, signal overlaps and low concentration. To overcome these and characterize Lac T2 relaxation in normal brain tissue at 7T (for the first time), this study used a MEGA-sLASER sequence with J-refocusing echo time extension. The measured T2 value of Lac was 182±10 ms in healthy human brain (occipital lobe), which can be used for absolute quantification of Lac. The measured T2 of NAA (169±8 ms) agrees with previous 7T reports. With high sensitivity, this approach showed its potential in detecting changes in Lac T2 in pathology.