Malignant ascites occurs in approximately 37% of terminal ovarian cancer patients. It significantly contributes to poor quality of life and mortality. Advances in understanding malignant ascites formation and finding new therapeutic options are urgently needed. High-resolution proton magnetic resonance spectroscopy provides opportunities to characterize biofluid metabolites and can be easily translated to the clinic. Here, we are investigating the metabolic profile of ascites obtained in two different experimental models of ovarian cancer. To further understand the differences observed between both models, we completed our study by analyzing the metabolic profiles of those cells in culture and their corresponding conditioned media.