Abstract #5605

Metabolomical NMR measurements of cell cultures: Increasing the metabolic stability of lysed cells by additional heating due to enzymatic inactivation.

Gaelle Diserens¹, Damian Hertig^2,3, Martina Vermathen², Balazs Legeza⁴, Christa E. Flueck⁴, Jean-Marc Nuoffer³, and Peter Vermathen¹

¹Depts. Radiology and Clinical Research, University Bern, Bern, Switzerland, ²Dept. Chemistry and Biochemistry, University Bern, Bern, Switzerland, ³University Institute of Clinical Chemistry, Bern University Hospital, Bern, Switzerland, ⁴Pediatric Endocrinology and Diabetology of the Dept. Pediatrics & Dept. Clinical Research, University Bern, Bern, Switzerland

Advanced NMR measurements of biological samples may take several hours. Preanalytical issues may impact the metabolite content, potentially leading to misinterpretation. Our aim was to investigate by ¹H HR-MAS NMR the impact of different cell handling preparation protocols (lysis with and without heating) on the stability of the cell metabolome over the measurement time. In lysed fibroblasts and adrenal-cells, metabolism was ongoing over-time, contrary to a stable metabolite content of the lysed-heated cells. Therefore, to minimize metabolome modifications over the measurement time, it is suggested to use cell lysis in combination with heat inactivation for extended HR-MAS NMR measurements.

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