Multiple sclerosis (MS) is a disease of the central nervous system associated with demyelination and glial activation affecting large areas of white matter. 1H-MRSI may detect the metabolic changes induced by MS and facilitate the differentiation between MS lesions. Our study aimed to detect and map the signal of macromolecules in healthy controls and MS patients. 2D FID-based 1H-MRSI was used with measurement time under 6 minutes. We found increased macromolecules in the perilesional region and decreased macromolecules in most of the lesions. However, in some lesions, macromolecules were increased, which may be possibly related to pathological activation of lesion.