The number of patients diagnosed with cancer is increasing. Therefore the ability to predict tumor response to therapeutic agents remains a major challenge. Tumor cell metabolism is currently examined by 1H MRS with the aim of getting more insight into the differences between normal and neoplastic tissues, characterize their metastatic potential/finding prognostic markers, and monitoring the effect of therapies. In this study we demonstrate the feasibility of characterizing in-vitro living cells and present changes in the metabolic profile of WM793 and T24 cells lines subjected to chemotherapy. These results show promise for more personalized treatment protocols for cancer patients.