A multislice method based on time-resolved imaging of contrast kinetics was developed for measurement of murine kidney size and function. A total of eight slices covering both kidneys were imaged with a temporal resolution of 1.23 sec/scan. The estimated kidney volume showed a good agreement with a three-dimensional volume scan. By fitting contrast kinetics using a modified bi-compartment model, renal perfusion and normalized glomerular filtration rate (GFR) were quantified, and subsequently used to calculate renal blood flow and GFR. In conclusion, this method allows simultaneous and reliable measurement of mouse kidney volume, perfusion, blood flow, and GFR.