Brain tissue can be modeled as a system with a water pool and an invisible pool of semi-solids. The exchange between these pools can be measured by saturating the semi-solids and observing the decrease in water signal after exchange. This requires making a differential measurement, comparing data with and without saturation. Here we present a variation of the STEAM sequence, adding water suppression, which allows for observation of exchanging spins in a single acquisition without a water background signal. The method was demonstrated in both a phantom and human subject at 3 T.