Inhibitory neurotransmitter GABA and antioxidant GSH are suggested to be implicated in psychiatric and neurological disorders. Because of their relatively weak signals, spectral editing is necessary to assess GABA and GSH in the human brain. Hadamard encoding can be applied simultaneously for spectral editing of GABA and GSH. As both small metabolite signals and Hadamard encoding are highly susceptible to frequency drift and motion, real-time frequency and motion correction significantly improves spectral quality. The data obtained in this study so far suggest good test-retest reliability of real-time frequency and motion corrected Hadamard encoded spectral editing (CHASE) for GABA and GSH.