Nicotinamide adenine dinucleotide (NAD+) is a ubiquitous molecule present in all cells and tissues of the body with an important role in the redox reactions and metabolism. Small changes in NAD+ levels may lead to oxidative stress and may be a cause for various disorders. NAD+ is usually be detected in vivo by 31P NMR spectroscopy. Recently, NAD+ measurement with 1H MRS in the human brain was demonstrated. In the present study, we show for the first time, in vivo single voxel localized 1H MRS detection of NAD+ from the human calf muscle at 7T.