One-dimensional projection method was applied to the simulation of spatially localized J-resolved magnetic resonance spectroscopy with real RF pulses. As a comparison, the same pulse sequence was simulated using non-localized ideal RF pulses. The resultant TE-averaged spectra of glutamate were compared with phantom experiment at 3T. Conspicuous differences between ideal pulse simulated spectrum and phantom spectrum were found. For vivo comparisons, metabolite quantification was performed with real RF pulse basis set and ideal pulse basis set, respectively. Real RF pulse generated basis set significantly improved the reproducibility of glutamate quantification in vivo.