Many cancer cells present an exacerbated glycolytic flux that provides advantage for growth and leads to extracellular acidosis. Dichloroacetate (DCA), a PDK inhibitor, shifts metabolism from glycolysis to glucose oxidation and decrease various cancer cells lines proliferation. However, as tumor cells are presenting metabolic plasticity, PDK inhibition may lack efficacy. To measure metabolic adaptations of cancer cells to acidic environment and in response to DCA, we studied metabolic fluxes using 13C-NMR spectroscopy. With this technology, we measured differences in metabolic profiles between parental cancer cells line and acidic clones and we quantified specific changes in metabolism following DCA treatment.