Abstract #1341

13C-NMR to study cancer cell metabolic plasticity following PDK inhibition. Influence of dichloroacetate and long-term exposure to acidic environment on glucose and glutamine metabolic pathways.

Céline Schoonjans¹, Nicolas Joudiou¹, Cyril Corbet², Olivier Feron², and Bernard Gallez¹

¹Biomedical Magnetic Resonance Group (REMA), Louvain Drug Research Institute, Catholic university of Louvain, Bruxelles, Belgium, ²Pharmacotherapy Group (FATH), Institute of Experimental and Clinical Research, Catholic university of Louvain, Bruxelles, Belgium

Many cancer cells present an exacerbated glycolytic flux that provides advantage for growth and leads to extracellular acidosis. Dichloroacetate (DCA), a PDK inhibitor, shifts metabolism from glycolysis to glucose oxidation and decrease various cancer cells lines proliferation. However, as tumor cells are presenting metabolic plasticity, PDK inhibition may lack efficacy. To measure metabolic adaptations of cancer cells to acidic environment and in response to DCA, we studied metabolic fluxes using ¹³C-NMR spectroscopy. With this technology, we measured differences in metabolic profiles between parental cancer cells line and acidic clones and we quantified specific changes in metabolism following DCA treatment.

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