Clinical feasible, comparable muscle MR-techniques are crucial for monitoring disease progression and therapy in patients with neuromuscular diseases. We developed and evaluated a multi-modal quantitative MR protocol at 3T. Diffusion parameters, water T2 relaxation time and fat-fraction were measured and tested for temporal stability, multicenter reproducibility and covariate influence. Diffusion parameters stabilized after 15 minutes and were comparable between centers. Water T2 decreased 1ms within 1 hour. In dorsal muscles fat-fraction increased slightly, due to a decrease in muscle size. Temporal stability of quantitative parameters was shown and showed that T2 decrease needs to be considered when planning protocols.