The purpose of this study was to examine the dependence of a quantitative texture metric, the high gray level run length emphasis (HGRE) in T2-weighted images, on common variations in image acquisition parameters. We studied 13 muscle disease patients with quantitative fat/water MRI and contrast-based images. The ability of the HGRE was unaffected by image matrix size. We also measured the dependence of the regression parameters on TR and TE. The results support the use of quantitative texture analysis to study clinically acquired MR images in muscle disease patients.