High angular resolution diffusion MRI (HARDI), the most widely used method in in-vivo brain imaging experiments to delineate white matter pathways, has been found sufficient for resolving 2-way fiber crossings but unstable for detecting 3-way fiber crossings. Therefore, if more sensitive and accurate tractography is wanted, researchers need to use high b-value with multi-shell q-ball models, which can be time-consuming. In this study, we compared 3 diffusion MRI acquisition protocols by tracking callosal connections between motor areas with both probabilistic and deterministic fiber tracking algorithms and provided a new scheme for the future diffusion MRI experiment.
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