Existing optic radiation (OR) tractography methods lack pediatric and surgical focus. We proposed a clinically feasible tractography framework and examined its test-retest reliability using both the preoperative and intraoperative MRI from eight pediatric epilepsy and brain tumor patients. Good to excellent intra- and inter-rater reproducibility was demonstrated in the assessments of all diffusion and morphological track metrics. The reconstructions closely resembled classic anatomy. All OR images were used to assist surgical planning and resection. Postoperatively, no patient had new visual field deficits. Our tractography method generates reproducible OR images that can be safely implemented in routine, non-emergency pediatric neurosurgical settings.