107 subjects were recruited between the ages of one month and 18 years. The study aimed to investigate the differences in the accuracy of five publicly available segmentation techniques on T1-enhanced and non-enhanced images compared to manual segmentation of the thalamus in a pediatric population. volBrain had the best outcomes in enhanced and non-enhanced images. Image segmentation using volBrain is the ideal methodology for thalamus segmentation. Gadolinium-enhancement negatively affects the outcomes of all the tested automated segmentation.