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Abstract #1812

Measurements of rat hippocampus Glu, Gln and GABA using NMR, MRS and HPLC in animal models of autism

Pawel Senator1, Elzbieta Zieminska2, Wojciech Hilgier2, Jaroslaw Orzel2, and Beata Toczylowska1,3

1Nalecz Institute of Biocybernetics and Biomedical Engineering Polish Academy of Sciences, Warsaw, Poland, 2Mossakowski Medical Research Center Polish Academy of Sciences, Warsaw, Poland, 3Institute of Biochemistry and Biophysics Polish Academy of Sciences, Warsaw, Poland

The goal of our studies was to compare different measuring methods of glutamine, glutamate and GABA of rat hippocampus used for study of pathogenesis of autism. The methods under consideration were: in vivo MRS and two in vitro ones, NMR and HPLC. Univariate statistical analysis of ratios of tested amino acids with respect to glutamate concentration was performed using General Linear Model. This demonstrated statistically significant differences between the results from three methods for both, glutamine and GABA ratios. OPLS-DA analysis allowed build models for differentiation of two animal models of disease and control group in NMR and HPLC.

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