HF subjects show brain injury in multiple areas, which may contribute to altered iron concentration in those sites. However, regional brain iron load in HF subjects is unclear. We examined regional iron deposition using R2*-relaxometry procedures and found altered R2*-values in the amygdala, brainstem, thalamus, globus pallidus, hippocampus, cerebellum, insula, and frontal and temporal white matter regions. The altered iron concentration in HF subjects may result from neural and white matter injury, including myelin and glial dysfunction, with iron potentially accelerating tissue degeneration. These data suggest that interfering with the iron action may reduce the exacerbation of injury in HF.