The purpose of this study is to investigate the relationship between CEST imaging and several neurodegenerative diseases to verify the feasibility of an estimation parameter derived from the CEST approach. For this study, patients with Parkinson’s disease, progressive supranuclear palsy and multiple system atrophy as well as healthy volunteers were examined. Region-of-interest analysis was performed in the substantia nigra and red nucleus area. As the results, the CEST parameters were significantly different for each of the neurodegenerative diseases and healthy volunteers. CEST imaging might have the ability to obtain more detailed information concerning neurodegenerative disease.