Multiple spin echo (MSE) imaging may enable improved quantification and characterization of tissue iron deposition, with application for assessment of liver iron overload. However, iron deposition generally results in non-exponential signal decay in MSE imaging, and MSE-based R2 (1/T2) relaxometry can depend on the inter-echo time. Additionally, it is cumbersome and expensive to empirically calibrate the R2 relaxometry-iron concentration relationship. In this work, we investigate the effect of inter-echo time on MSE signal in the presence of liver iron overload using Monte Carlo simulations. This Monte Carlo approach may enable improved calibration of MSE-based measurements of iron concentration.