Ex-vivo lung perfusion and ventilation systems are a promising new tool for conditioning marginal lung allografts. However, reliable biomarkers for evaluating graft function are missing. In this study MRI-derived fluorine and oxygen washout times are to be evaluated as lung function parameters in a porcine model of ischemia. Washout time for oxygen is prolonged while fluorine washout is not in lungs after warm ischemia compared to normal controls, which might reflect pulmonary edema limiting oxygen diffusion. Determination of fluorine and oxygen washout is feasible in an ex-vivo lung perfusion system and seems to be promising tools for evaluating graft function.