R1ρ (=1/T1ρ) imaging has been applied in many human organs to characterize tissue biochemical changes. However, R1ρ imaging in human kidney has been rarely reported partly due to the challenges associated with field inhomogeneities and respiratory motion. We developed an R1ρ imaging protocol for human kidney which used adiabatic half passage pulse and volume shimming to overcome field inhomogeneities. In addition, R1ρ dispersion was evaluated via a simple method with a fixed locking time but different locking frequencies. The volunteer scans exhibited characterized R1ρ maps in kidney, also there was greater R1ρ dispersion between locking frequencies of 100Hz and 300Hz.