PDFF and T2* have been previously proposed as two important parameters in quantitative MRI of adipose tissue. This study investigates the difference between gluteal and supraclavicular adipose tissue T2* and the relationship between adipose tissue T2* and PDFF using a twenty-echo multi-echo gradient echo acquisition. A highly significant difference between the PDFF in different fat regions was detected in water-fat separation results when using either the first 6 echoes or the full 20 echoes. However, T2* values were only significantly different between fat regions, when using the full 20 echoes and not when using the first 6 echoes. PDFF also correlated with T2* when using the full 20 echoes.