The impact of left ventricular (LV) ejection fraction (LVEF) on cardiovascular blood flow is not completely understood. We used a method, called “Atlas heart generation”, to investigate cardiovascular flow of patients with ischemic heart disease (n=62). The patients underwent 4D-Flow MRI and were stratified according to LVEF. We found that the lower LVEF group had lower velocities throughout the aorta, in a portion of LV and left atrium, at peak-systole. At early-diastole, differences were observed in the aortic arch, and in the apical-septal segments of LV. The suggested method can detect changes in cardiovascular flow and add to pathophysiological understanding.