Elevated pulse wave velocity (PWV) is a measure of aortic stiffness and an indicator of cardiovascular disease. Pulse waves propagate quickly along the aorta and high-temporal resolution measurement of velocity data with full spatial coverage is needed to improve the precision of PWV estimation. This study used high-temporal resolution 4D flow MRI to assess PWV and investigate the impact of temporal resolution on PWV estimation methods (i.e. time-to-foot and cross-correlation) in patients with known atherosclerosis. The findings suggest that using cross-correlation to estimate the time-delay between flow waveforms is optimal, particularly at inferior temporal resolutions.