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Abstract #3069

Monitoring effect of rapamycin on pyruvate metabolism in SCC tumor using hyperpolarized 13C-MRI

Keita Saito1, Shingo Matsumoto1,2, Yoichi Takakusagi1,3, Masayuki Matsuo1,4, Hellmut Merkle5, James B Mitchell1, and Murali C Krishna1

1National Cancer Institute, Bethesda, MD, United States, 2Hokkaido University School of Engineering, Sapporo, Japan, 3National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan, 4Gifu University School of Medicine, Gifu, Japan, 5National Institute of Neurological Disorder and Stroke, Bethesda, MD, United States

Effect of an mTOR inhibitor rapamycin on pyruvate metabolism in squamous cell carcinoma (SCC) xenografts was investigated using hyperpolarized 13C-MRI. [1-13C]lactate to [1-13C]pyruvate ratio (Lac/Pyr) in the SCC tumors increased as tumor grew in non-treated control mice, whereas it significantly dropped after 2 days of the rapamycin treatment. Inhibition of mTOR caused a drop of LDH protein level and the activity in the SCC tumor, and perfusion in the tumor was improved by the rapamycin treatment. Lac/Pyr monitored using hyperpolarized 13C-MRI would become a useful marker for tumor response to mTOR inhibitors.

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