Diffusion MRI is routinely used in prostate cancer diagnosis. Diffusion kurtosis imaging allows measuring the kurtosis Kapp, related to deviations from free diffusion, additionally to the diffusion coefficient Dapp. Varying the diffusion time may yield additional information about the investigated tissue by probing the diffusion barriers at different length scales. Here, Dapp and Kapp were measured at three diffusion times in 27 patients with histologically confirmed prostate cancer. A reduction of Kapp was observed in tumor and normal control regions with increasing diffusion time, while a Dapp reduction was mostly seen in control regions.