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Abstract #3585

Sensing intracellular calcium ions using a manganese-based MRI contrast agent

Benjamin B Bartelle1, Barandov Ali1, Catherine G Williamson1, Emily S Loucks1, and Alan P Jasanoff1

1Bioengineering, Massachusetts Institute of Technology, Cambridge, MA, United States

Inspired by the classical work of R. Tsien on cell-permeable calcium specific chelators using readily cleavable acetomethoxy esters (AM) of BAPTA and taking advantage of our own, novel, cell permeable manganese-based contrast agent, we developed a new MRI contrast that displays membrane permeability, cellular accumulation via cleavable ester groups, and physiological sensitivity to calcium. With this breakthrough innovation, we present a series of firsts for functional molecular imaging, reporting chemically and optogenetically induced calcium transients with MRI in living cells.

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