Amplitude of low-frequency fluctuations (ALFF) of blood oxygenation is a marker of resting-state functional magnetic resonance imaging (fMRI) used to measure local spontaneous activity of the brain. However, interpretation of ALFF results is still unclear. Comparing ALFF values in left and right temporal-lobe epilepsy (TLE) patients with controls showed an increase in seizure-related regions. Increases in cerebral blood flow (CBF) were also found to be partially responsible for the increase in ALFF in another cohort of controls. Therefore, the development of ALFF measures may potentially provide a non- invasive perfusion measure in the presurgical evaluation of TLE.