Chemical warfare nerve agents induce neurological damage through seizures. It is widely accepted in the field of nerve agent research that excitotoxicity is the main contributor to the neuropathology. Growing evidence suggests the involvement of hypoxia in seizure-related neuropathology. However, it is difficult to detect hypoxia without directly measuring oxygenation. We applied a method we developed to simultaneously quantify tissue oxygenation and cerebral blood flow to detect hypoxia following soman-induced seizures.