Glaucoma is a neurodegenerative disease that can cause irreversible vision loss. Elevated intraocular pressure is a major risk factor for the glaucoma; however, the disease may still progress in some patients after lowering IOP. Citicoline has been suggested as a potential therapeutic to ameliorate damage caused by neurodegenerative diseases, including glaucoma, but its neuroprotective effects remain incompletely studied. In this study, we analyzed the dose-dependent effect of oral citicoline on visual behavior response and white matter integrity in a rodent model of glaucoma. The results show citicoline preserves visual behavior response and visual system integrity in a dose dependent manner.