Focused Ultrasound in conjunction with microbubbles allows the reversible and safe opening of the BBB in a narrow range of Acoustic Pressure (AP). Here we wanted to investigate what happens to the brain tissues at short- and long-terms in case of an excessive AP is reached in vivo. Passive Cavitation Detection has been used to register inertial cavitation during the opening. At 1,3,7,14 and 28 days after the BBB opening, T1,2-weighted images, T2*-maps, DWI, MRS acquisitions and histological analysis have been performed. P-gp and myelin expression, cells deaths, and astrocytes/microglial activations have been also imaged postmortem within 28 days.