High field Magnetic Resonance Angiography on mouse eye can be used to investigate the vasculature near the mouse eye. One of the difficulties with using on mice is the induced moving artifact. To overcome this obstacle, a custom mouse restrainer containing both a tooth bar and ear bars was designed and constructed for mouse eye imaging. The restrainer, in combination with a rigid surface coil, enabled three-dimensional mouse eye images to be captured with 30 um isotropic resolution. A three-dimensional mouse angiography was created by segmenting ocular blood vessels from these images.