To advance hyperpolarized Xenon-129 MRI as a robust tool for probing pulmonary gas-exchange in lung diseases, establishing repeatability of quantitative ratios and regional metrics is critical. Repeatability was assessed using Bland-Altman analysis. Average inter-visit tissue-to-gas, red blood cell-to-gas (RBC-to-gas) and RBC-to-tissue ratios were highly repeatable; the primary source of variation was due to lung inflation volume variations, suggesting consistency of breath-hold coaching and dose delivery is important. Regional distribution of measures was also qualitatively well-matched between visits. Demographic factors may also influence these measures; our preliminary data indicate a significant relationship between RBC-to-tissue ratio and subject age.