Obesity and overweight are common problems in developed world population, affecting to a greater number of children. In young people, it is becoming more usual to diagnose diabetes or heart problems due to fat accumulation. In this work, we wanted to assess the effects of a fat diet in the cerebral response to an appetite stimulus in mice. We evaluated changes in diffusion magnetic resonance imaging parameters and metabolomic profiles. Our results suggest that high fat diet consumption causes alterations in the brain response to appetite that can be signaled by magnetic resonance approaches.