Aquaporin-4 (AQP4) is the main water channel protein found in the brain. It is located at the end-feet of astrocytes allowing to maintain the ion and water balance. In our group, we previously employed functional diffusion magnetic resonance imaging to detect the swelling of astrocytes in response to appetite. In this line, we wanted to study the function of the AQP4 in the volume changes of these glial cells. For that, we administrated a glucose bolus and the aquaporin inhibitor TGN to assess the role of this protein in the brain response to a feeding stimulus.