Diagnosis of high grade serous ovarian cancer (HGSOC) requires a biopsy which is both invasive and does not reflect the heterogeneity of the disease. Diffusion kurtosis imaging (DKI) was performed on 23 treatment naïve ovarian cancer patients. Both mean Dapp (apparent diffusion) and mean Kapp (apparent kurtosis) were found to be significantly different in HGSOC compared to other types of epithelial cancer. Kapp was also significantly greater in the patients who went on to respond to chemotherapy treatment compared to non-responders. DKI may therefore aid in identifying HGSOC and in the selection of the best treatment for individual patients.