Flux through pyruvate dehydrogenase, a key regulatory enzyme, may be detected in human myocardium by hyperpolarization (HP) technology. A previously-validated model for quantitation of pyruvate metabolism in isolated hearts was extended to human myocardium. HP [13C]bicarbonate and HP[1-13C]lactate were detected from heart muscle after injection of ~0.43 mL/kg of 250mM HP[1-13C]pyruvate in four healthy subjects. All subjects tolerated the procedure well. Kinetic modeling yielded a rate constant for oxidation of pyruvate to bicarbonate of 0.005 sec-1, lower than that reported for isolated rodent hearts. Assessment of PDH flux in human myocardium is feasible with hyperpolarization technology.