Previous approaches on left ventricle segmentation from DE-MRI have focused on the extraction of myocardium or just diseased region in short axis orientation. However these studies are not well suited for the segmentation of non-diseased myocardium region on DE-MRI. This paper presents a novel semi-automatic segmentation method of non-diseased myocardium region segmentation on diseased patient heart from DE-MRI based on watershed algorithm with shape priors application. To assess the results segmented images were compared with gold standard images performed by an experienced user. Novel solution for DE-MRI segmentation has been proposed, which brought promising results of measured parameters.