The goal of this study was to apply high-resolution diffusion weighted imaging (DWI) at 7T to assess depth dependence of diffusion in the articular cartilage in the knee. Four healthy volunteers were scanned with a diffusion prepared TSE sequence. ADC maps were consequently used to assess the depth dependence of ADC throughout the articular cartilage. The ADC value was shown to significantly increase from the bone-cartilage interface to the superficial zone. This work shows that the significant depth dependence of the ADC in cartilage layers can be observed in vivo on 7T with diffusion weighed imaging.