Quantitative magnetization transfer (qMT) imaging was used to measure bound water fraction in mouse kidneys with renal artery stenosis (RAS). MT-weighted images at variable offset frequencies and amplitudes, as well as B0, B1, and T1 maps of control and RAS kidneys, were acquired. A two-pool qMT model was used to estimate the bound water fraction as well as other relaxation and exchange parameters. An increased bound water fraction was found in the cortex, outer medulla, and inner medulla of the RAS kidneys. In conclusion, qMT imaging offers potential new biomarkers for assessment of RAS kidneys.