We evaluated the usefulness of non-contrast perfusion obtained using arterial spin labeling (ASL) for the assessment and the differential diagnosis of shoulder disorders. Twenty consecutive patients were divided into 4 groups. Group 1 (partial tear or less) had no enhancement at the subacromional region; whereas, group 2 (full thickness tear of rotator cuff) showed significant enhancement due to bursitis. Group 3 (calcific tendinitis) and group 4 (osteoarthritis) also showed well enhancement. ASL perfusion could provide the additional physiological information to the morphological routine shoulder MRI without use of contrast materials, which is valuable for the management of shoulder disorders.